Chromosomal origin of replication coordinates logically distinct types of bacterial genetic regulation
نویسندگان
چکیده
منابع مشابه
A hybrid bacterial replication origin.
We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resi...
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ژورنال
عنوان ژورنال: npj Systems Biology and Applications
سال: 2020
ISSN: 2056-7189
DOI: 10.1038/s41540-020-0124-1